Bio-Rad electrophoresis cell

The electrophoresis system consisting of a power module from Bio-Rad, type CHEF-DR III, and an electrophoresis cell from Bio-Rad, constitue the CHEF-DR III Variable Angle System.
Pulsed field electrophoresis is a technique for resolving chromosome-sized DNAs. By alternating the electric field between spatially distinct pairs of electrodes, megabase (mb) sized DNAs are able to reorient and move at different speeds through the pores in an agarose gel.
The CHEF-DR III system separates large and small DNA fragments with better resolu- tion, speed, and accuracy, than initial pulsed field methods. DNAs from 100 bases to over 10 megabases (mb) may be effectively resolved. For example, the chromosomal DNA of Schizosaccharomyces pombe can be resolved in 1 day using a 106° pulse angle, compared to 2 days at 120°. Everything from Yeast Artificial Chromosomes (YACs) to M13 inserts can be separated with a single instrument. Applications inclutop down and bottom up map- ping (Not I and cosmid cloning, respectively), electrophoretic karyotyping, analysis of tumor cell DNA rearrangements, mammalian DNA analysis, and testing for bacterial, yeast, and parasite strain homogeneity.
Technical specifications:
Voltage: 220-240V, 50/60Hz
Power: 300W
External dimensions (W) x (D) x (H): 45 x 57 x 10 cm